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ER-HR3 (Biotin)

ER-HR3 (Biotin)

Cat no: E3566-01B

Supplier: United States Biological
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ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in hematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low. During fetal development, ER-HR3 positive cells are localized to hemapoietic islands and are often associated with erythroid progenitor cells. The functions of the ER-HR3 antigen have not been established but reports suggest that the antigen may be involved in adult erythropoiesis and in the regulation of the immune response. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3566-01B
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG2c
Purity: Purified by Protein G affinity chromatography.
References: 1. de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages.II. Biochemical and functional aspects of the ER-HR3 antigen. Cell Tissue Res. 275:577
Additional info: Recognizes mouse antigen ER-HR3. Does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies. Recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions.

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