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ER-HR3

Cat no: E3566-01


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3566-01
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry
Size: 250ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG2c
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes mouse antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in hematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low.

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