ERBB2, Recombinant, Human (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL)

HER-2/neu (erbB-2) encodes an 185kD orphan receptor tyrosine kinase that is constitutively active as a dimer and displays potent oncogenic activity when overexpressed. Herstatin, as the product of alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage- independent growth of transformed cells that overexpress HER-2.\n\nAmino Acid Sequence:\nMTQVCTGTDM KLRLPASPET HLDMLRHLYQ GCQVVQGNLE LTYLPTNASL SFLQDIQEVQ GYVLIAHNQV RQVPLQRLRI VRGTQLFEDN YALAVLDNGD PLNNTTPVTG ASPGGLRELQ LRSLTEILKG GVLIQRNPQL CYQDTILWKD IFHKNNQLAL TLIDTNRSRA CHPCSPMCKG SRCWGESSED CQSLTRTVCA GGCARCKGPL PTDCCHEQCA AGCTGPKHSD CLACLHFNHS GICELHCPAL VTYNTDTFES MPNPEGRYTF GASCVTACPY NYLSTDVGSC TLVCPLHNQE VTAEDGTQRC EKCSKPCARG THSLPPRPAA VPVPLRMQPG PAHPVLSFLR PSWDLVSAFY SLPLAPLSPT SVPISPVSVG RGPDPDAHVA VDLSRYEG\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 0.1M Acetate buffer, pH 4.0. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3451-25R

Size

20ug

Applications

ELISA, WB

Form

Supplied as a lyophillized powder from 0.05mM Acetate buffer, pH 4. Reconstitute with 0.1M Acetate buffer, pH 4.0 at 10ug/ml. Further dilutions can be made in desired buffer.

Purity

Purified (~95%)

References

1. Jhabvala-Romero F, (2003) Oncogene. 22(50): 8178-86. 2. Azios NG (2001) Oncogene. 20(37): 5199-209. 3. Doherty JK, (1999) Proc Natl Acad Sci USA. 96(19): 10869-74.