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ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3) (BSA & Azide Free)

Cat no: E3451-35EX

ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3) (BSA & Azide Free)

c-erbB-3/HER-3 is the third member of the type I family of growth factor receptors. It binds to ligands in the heregulin family. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas, and colon. Heregulin & EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. \n\nMW of Antigen: 185kD \n\nEpitope: aa1234-1259\n\nApplications and Working Dilutions: \nImmunoprecipitation (Denatured verified) (Use Protein G) (Ab 2ug/mg protein lysate) \nWestern Blotting (Ab 1-2ug/ml for 2hrs at RT) The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator. \n\nPositive Control: T47D, SKBR3 or MAD109 cells. \n\nCellular Localization: Cytoplasmic and cell membrane\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3451-35EX

Size

100ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See E3451-35E

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Prigent SA and Lemoine NR. (1992) Progress in Growth Factor Research 4:1

Additional Info

For optimum results, immunoprecipitate with erbB-3 and Western blot Species Crossreactivity: human, rat, mouse.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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