ErbB3, phosphorylated (Tyr1289) (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

HER3/ErbB3 is a member of the ErbB/EGF receptor protein tyrosine kinase family (1,2). Tyrosine phosphorylation of HER3/ErbB3 depends on its association with other ErbB tyrosine kinases (3). Phosphorylation sites in the carboxy-terminal tail mediate downstream signaling\npathways. HER3/ErbB3 is highly expressed in many cancer cells (4), and it may work together with other Erb members in tumorogenesis and proliferation (5). HER/ErbB3 is phosphorylated by EGFR or ErbB2 through dimeric interactions with HER/ErbB3, on Tyrosine 1289, a docking\nsite for phosphatidylinositol 3-kinase (6). \n\nApplications: \nSuitable for use in Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 1:100\nWestern Blot: 1:2500-1:5000\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

E3451-36E

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

1. Katoh, M., et al. Biochem Biophys Res Commun. 192: 1189 2. Kraus, M.H., et al. Proc Natl Acad Sci U S A. 86: 9193 3. Guy, P.M., et al. Proc. Natl. Acad. Sci. USA 91: 8132 4. Sithanandam, G., et al. Carcinogenesis 24: 1581 5. Alimandi, M., et al. Oncogene 10: 1813 6. Jie Zhang, The American Journal of Pathology 170(1), 2007

Additional Info

Recognizes human HER3/ErbB3 phosphorylated on Tyrosine 1289.\n

Alternative Names

v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (avian), HGNC:3431, ErbB-3, MDA-BF-1, c-erbB-3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3