ErbB4 (c-erbB4, HER4)

c-erbB-4 is the fourth member of class I receptor kinase family (ErbB). HER-4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDA-MB- 330 and MDA-MB-361. Expression of erbB-4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3.\n\nApplications:\nSuitable for use in ELISA. Other applications have not been tested. \n\nRecommended Dilutions:\nOptimal dilutions to be determined by researcher. \n\nPositive Control: T47D or MDA-MB-453 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3451-40E

Size

250ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Chen X, et. al. (1996) J Biol Chem 271(13):7620-9. 2. Tang C, et. al. (1999) Cancer Res. Oct 15; 59(20):5315-22. 3. Mason S., and Gullick WJ. (1995) Breast 4:11-18. 4. Hynes, N.E., Stern, D.F. (1994) Biochim. Biophys. Acta 1198:165-84. 5. Peles, E., and Yarden., Y. (1993) Bioessays 15:815-24. 6. Lupu, R., et al., (1995) seminars in Cancer Biology 6:135-145. 7. Dougall, W.C., et al., (1994) Oncogene 9:2109-2123. 8. Earp, H.S., Dawson, T.L., Li, X., and Yu, H. (1995) Breast Cancer Research and Treatment 35:115-132. 9. Rajkumar, T. and Gullick, W. J. (1994) Breast Cancer Res Treat 29:3-9. 10. Marchionni, M.A. (1995) Nature 378:334-335. 11. Carraway, K.L., III. and Burden, S.J. (1995) Current Opinion in Neurobiology, 5:606-612. 12. Prigent SA and Lemoine NR., (1992) Progress in Growth Factor Research 4:1-24. 13. Ben-Baruch N; Yarden Y. (1994) Proceedings of the Society for Experimental Biology and Medicine 206(3):221-7. 14. Carraway KL 3rd, et al., (1994) Cell 78(1):5-8. 15. Tzahar E, et al., (1994) J. Biol. Chem. 269:25226-25233. 16. Beerli, R.R., et al., (1995) Mol. Cell. Biol., 15:6496-6505. 17. Plowman, G. D., et al., (1993) Proc. Natl. Acad. Sci. U. S. A. 90. 18. Culouscou et al., (1993) Journal of Biological Chemistry 268:18407-10. 19. Meyer, D., et al. (1995) Nature 378:386-390. 20. Riese, D.J.II., et al., (1995) Mol. Cell. Biol., 15:5770-5776. 21. Riese II D J., et al., (1996) Oncogene 12:345-353. 22. Gassmann, M., et al., (1995) Nature 378:390-394. 23. Meyer, D. and Birchmeier, C. (1995) Nature 378:386-390.

Additional Info

Recognizes human c-erbB4. Species Crossreactivity: mouse and rat.