ErbB4 (c-erbB4, HER4) (PE)

ErbB4 is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. However, ErbB4 has also been shown to heterodimerize with both ErbB1 and ErbB3. It has been suggested that the identity of the ligand may influence the dimerization partner. Several ErbB4 isoforms exist. Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme, ADAM17) to proteolytically cleave ErbB4 from the cell surface. These isoforms exhibit tissue-specific expression. Another isoform lacks the phosphoinositide 3-kinase activation sequence present in the ErbB4 cytoplasmic domain. Human ErbB4 consists of 1308 amino acids (aa) with a 25aa signal sequence, a 626aa extracellular domain, a 24aa transmembrane region, and a 633aa cytoplasmic domain. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels up to 1x10e6 cells or 50ul of packed whole blood.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C0015-01Q

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Plowman, G.D. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1746. 2. Elenius, K. et al. (1997) J. Biol. Chem. 272:26761. 3. Elenius, K. et al. (1999) Oncogene 18:2607. 4. Rio, C. et al. (2000) J. Biol. Chem. 275:10379. 5. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172. 6. Sundaresan, S. et al. (1998) Endocrinology 139:4756. 7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859. 8. Schlessinger, J. (2000) Cell 103:211. 9. Daly, R.J. (1999) Growth Factors 16:255.

Additional Info

Recognizes human ErbB4.