The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.
Applications:
Suitable for use in Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:100-1:200, In an ABC method, we suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining.
Optimal dilutions to be determined by the researcher.
Positive Control:
Tonsil.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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