ERK-1/2, phosphorylated (Thr202, Tyr204)

Mitogen activated protein kinase (MAPKs) mediate signal transduction cascades from the cell surface to the nucleus that are responsible for cell growth and differentiation. The activity of MAPK family members is dependent upon dual phosphorylation of threonine and tyrosine residues by an upstream kinase, MKK (or MEK). In mammals, three types of MAPK pathways have been identified: the ERKs (extracellular signal-related protein kinases), JNKs (c-Jun NH2-terminal kinases), and p38 MAP kinases. Activation of a MAPK signaling cascade requires a minimum of three events: the phosphorylation of a MAP kinase kinase kinase (MKKK or MEKK) triggers the phosphorylation of a MAP kinase kinase (MKK or MEK), which in turn phosphorylates the MAP kinase. ERKs are regulated by the binding of extracellular ligands to transmembrane receptors possessing intrinsic or associated tyrosine kinase activity. At least four distinct ERKs have been identified, including ERK1 (p44), ERK2 (p42), ERK3 (p62), and ERK4 (p45). In humans, activation of MAP kinase (ERK1+ERK2) requires phosphorylation by MEK at Thr202 and Tyr204.1\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot (rat): 1:1000. \nImmunohistochemistry, human (formalin fixed, paraffin embedded): 1:400 Staining of formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval with citrate buffer, pH 6.0, is required prior to staining.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E3452-57

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM Hepes solution, pH 7.5, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified.

Isotype

IgG

References

1. Payne DM, et al. (1991) EMBO J 10(4):885-892.

Additional Info

Recognizes ERK 1+2 when phosphorylated at Thr202 and Tyr204. On Western blots, it identi?es two bands at ~42 and 44kD. Reactivity has been con?rmed with UV-treated human Jurkat cell lysates. Immunolabeling in UV-treated Jurkat cells is blocked by the phosphopeptide, but not by the dephosphopeptide.