Erk1/2, diphosphorylated (Extracellular Signal-regulated Kinase 1, ERK1, ERK-1, ERK, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERT1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p42, MAP Kinase Isoform p44, Microtubule-associated P

Extracellular signal regulated kinase (ERK), also known as mitogen activated protein kinase (MAPK), is a family with two homologous members; ERK1 (44kD) and ERK2 (42kD). This antibody reacts with the active forms of these proteins that have specific phosphorylated Threonine and Tyrosine residues within a Thr-Glu-Tyr motif (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2)\n\nApplications: \nSuitable for use in Western Blot . Other applications not tested. \n\nRecommended Dilution: \nWestern Blot:. 0.25-0.50ug/ml. Band expected at ~43kD for human Erk1 and ~41kD for human Erk2.\nOptimal dilution determined by the researcher. \n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3452-45D1

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Yeast/Fun

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with 1ml sterile PBS.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes the epitope containing phosphorylated threonine and tyrosine residues within the regulatory site of active human ERK1 and ERK2 (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2). Species Crossreactivity: mouse, rat, yeast.