ERK1 (Extracellular Signal-regulated Kinase 1, ERK-1, Esrk1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, Mapk3, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, MAPK1, Mi

Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on speci?c threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identi?ed and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family are the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identi?ed including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggest that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins including: RSK (4) , c-Fos, c-Jun, c-Myc, c-raf, MAP2, and MEK (5) . MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by many factors (6,7,8) .\n\nApplications: \nSuitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1

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SPECIFICATIONS

Catalog Number

E3452-45F

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Peptide-speci?c af?nity chromatography.

Isotype

IgG1,k

References

1. Marshall, C.W., et al; Cell 80:179-185 (1995).\n2. Herskowitz, I., et al; Cell 80:187-197 (1995).\n3. Cobb, M.H., et al; Cell Regulation 2:965-978 (1991).\n4. Sturgill, T.W., et al; Nature 334:715-718 (1988).\n5. Matsuda, S., et al; J Biol Chem 267:3277-3281 (1993).\n6. Ward, et al; Nature 367:651-654 (1994).\n7. Boulton, T.G., et al; Cell 65:663-675 (1991).\n8. Crews, C.M., et al; Science 258:478-480 (1992).\n9. Waddell, T.K., et al; J. Biol. Chem. 270(25):15403-15411 (1995).\n10. Gardiner, E.E., et al; J. Biol. Chem. 274 (17):11930-11936 (1999).\n11. Kawakami, Y., et al; Proc. Natl. Acad. Sci. 94:3938-3942 (1997).

Additional Info

Speci?c for ERK1 and does not crossreact with ERK2 or related molecules. Reactivity has been con?rmed by western blot analysis of extracts derived from human A431 and K562 cells, canine MDCK cells, mouse brain, rat brain and recombinant Erk2 (rec-ERK2 was negative). USAGE The dilutions below are only starting recommendations. Optimal concentrations of this antibody should be determined by the researcher for each speci?c application.

Alternative Names

EC=2.7.11.24