Erk1 (Extracellular Signal-Regulated Kinase 1, ERK-1, P44ERK1, p44-ERK1, ERT2, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, P44MAPK, p44-MAPK, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, MAPK3, Microtubule-asso

ERK1, a MAPK-type protein kinase, is a member of the Ras-MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins, and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK1 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK1 causing dissociation of the MEK/ERK complex. A portion of the activated ERK1 pool forms dimers and translocates to the nucleus to activate nuclear proteins such as ELK-1 and MSK1. Cytosolic ERK 1 acts on a different set of substrates, including MNK1/2 and MAPKAP-K1.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 7ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman brain, neurons and glia\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E3452-44E

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. J Biol Chem 2006 Nov.: Cell type specific effect of hypoxia and PDGF-BB on extracellular matrix turnover and its consequences for lung remodelling. Karakiulakis G, Papakonstantinou E, Aletras AJ, Tamm M, and Roth M. 2. Am J Respir Cell Mol Biol 2006 Nov.: Cigarette Smoke Stimulates Matrix Metalloproteinase-2 Activity via EGR-1 in Human Lung Fibroblasts. Ning W, Dong Y, Sun J, Li C, Matthay MA, Feghali-Bostwick CA, and Choi AM. 3. Int Immunol 2006 Nov.: Effects of TREM-1 activation in human neutrophils: activation of signaling pathways, recruitment into lipid rafts and association with TLR4. Fortin CF, Lesur O, and Fulop T. 4. Biochem Pharmacol 2006 Oct.: Selective cyclooxygenase-2 inhibitors stimulate glucose transport in L6 myotubes in a protein kinase Cdelta-dependent manner. Alpert E, Gruzman A, Tennenbaum T, and Sasson S. 5. Life Sci 2006 Oct.: Involvement of protein kinase Cdelta in iron chelator-induced IL-8 production in human intestinal epithelial cells. Choi EY, Lee S, Oh HM, Kim YD, Choi EJ, Kim SH, Kim SW, Choi SC, and Jun CD.

Additional Info

Recognizes human ERK1/MAPK3.

Alternative Names

EC=2.7.11.24, MGC20180