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ERK5 (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7)

Cat no: E3452-61E

ERK5 (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7)

Erk5 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals. They are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Erk5 kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. This transcript variant (3) is also known as BMK1 gamma. It contains a different 5' UTR, and encodes the identical isoform (1), as compared to variant 1.\n\nApplications: \nSuitable for use in Western Blot. May be suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1906: IgY, Chicken Pab Rb x Ch \nI1906-10: IgY, Chicken-HRP Pab Rb x Ch \nI1906-12: IgY, Chicken-FITC Pab Rb x Ch \nI1906-14: IgY, Chicken-AP Pab Rb x Ch\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3452-61E

Size

50ug

Applications

WB

Hosts

Ch/Bird

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. Glycerol free.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

1. Kato, Y., et al., Nature 395(6703): 713-716 (1998).

Additional Info

Recognizes human Erk5.

Alternative Names

EC=2.7.11.24

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