ERO1L, CT (ERO1L, ERO1-like protein alpha, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha)

Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

035218

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Inaba, K., et al. EMBO J. 29(19):3330-3343(2010)\nAppenzeller-Herzog, C., et al. EMBO J. 29(19):3318-3329(2010)\nSwiatkowska, M., et al. J. Biol. Chem. 285(39):29874-29883(2010)\nChambers, J.E., et al. J. Biol. Chem. 285(38):29200-29207(2010)\nRose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Additional Info

Human

Alternative Names

ERO1L, ERO1-like protein alpha, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha