Erythropoietin Receptor (EPOr)

The Human Erythropoietin Receptor (EPOr) is located on stem and mature cells of the hematopoietic system. When activated by its native ligand, Erythropoietin, EPOr mediate proliferation and maturation of the erythroid precursor cells leading to expansion of red cell populations. Human recombinant EPO is currently used for treatment of patients undergoing erythrocyte depletion due to dialysis for kidney failure. \n\nApplications: \nSuitable for use in Immunoprecipitation, Western Blot, Neutralization, Immunohistochemistry, Immunocytochemistry and ELISA. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: 10ug/ml\nWestern Blot: 10ug/ml\nNeutralization: 500ug/ml. Neutralization of ligand binding has been detected using erythroleukemia cell line TF-1 with 3000 Epo receptors/cell to block Iodine 125 labeled erythropoietin ligand binding. \nImmunohistochemistry: 10ug/ml\nImmunocytochemistry: 10ug/ml\nELISA: 50ng/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-59C: IgG, H&L (HRP) Pab RbxSh\nI1904-59HI: gG, H&L, X-Adsorbed (HRP) Pab RbxSh\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3455-11

Size

50ug

Applications

ELISA, ICC, IHC, IP, WB

Hosts

Sheep

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Nakatsuji T, (2005) Haema 8(3):450-457. 2. Fairchild Benyo D, Conrad KP. et al., (1999) Biol Reprod. Apr;60(4):861-70. 3. Harris KW, et al., (1992) J Biol Chem. Jul 25;267(21):15205-9.

Additional Info

Recognizes human EPOr at 49kD at concentrations of 50ng/ml. Species Crossreactivity: mouse.