Escherichia Coli Elongation Factor TU (EF-TU)

Elongation factor Tu (EF-Tu) is a 43kD multifunctional protein present in Escherichia coli. EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins. Monoclonal antibody 900 recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes.\n\nApplications: \nSuitable for use in ELISA, Immunofluorescence and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:50\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2213-94

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1.Weber, S et al; An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains. J Bacteriol 1995, 177: 11 2.Baensch, M et al; Conservation of the amino-terminal epitope of elongation factor Tu in eubacteria and archaea. Microbiology 1998, 144: 2241 3.Kunert, A et al; Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein. J Immunol 2007, 179: 2979

Additional Info

Recognizes Escherichia Coli Elongation Factor TU at the very end of the N-terminus of the EF-Tu molecule (SKEKFE) at ~43-47kD (Bacterial), ~60 or ~80kD (archael). Species Crossreactivity: Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, Deinococcus sp., and weakly crossreacts with Mycobacterium tuberculosis.