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Escherichia coli, J5 LPS (E. coli)

Escherichia coli, J5 LPS (E. coli)

Cat no: E3500-28J

Supplier: United States Biological
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The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella). Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E3500-28J
Hosts: Mouse
Applications: ELISA, Immunofluorescence
Size: 100ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
Additional info: Recognizes E. coli J5 LPS. Species crossreactivity: K. pneumoniae, S. sonnei and S. typhimurium.

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