Escherichia coli, O, K Serotypes (E. coli) (Biotin)

Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nTiter: >1:2000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 and K12\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3500-06D

Size

1ml

Applications

ELISA

Hosts

Rabbit

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added. Labeled with Biotin.

P Type

Pab

Purity

Purified IgG fraction to a purity of >95%.

Isotype

IgG

References

General References:\n1. \"Escherichia coli O157:H7\". CDC Division of Bacterial and Mycotic Diseases. Retrieved on 2007-01-25.\n2. Vogt RL, Dippold L (2005). \"Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002\". Public Health Rep 120 (2): 174

Additional Info

Recognizes all \"O\" and \"K\" antigenic serotypes of Escherichia coli. Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae. Will remove E. coli proteins from recombinant preparations.