Escherichia coli, O157 (E. coli)

Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.\n\nApplications: \nSuitable for use in Immunofluorescence and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:10-1:50\nELISA: 1:20-1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3500-42

Size

1ml

Applications

ELISA, IF

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG3

References

1. \"Escherichia coli O157:H7\". CDC Division of Bacterial and Mycotic Diseases. Retrieved on 2007-01-25. 2. Vogt RL, Dippold L (2005). Public Health Rep 120 (2): 174-8. PMID 15842119. 3. Bentley R, Meganathan R (01 September 1982). Microbiol. Rev. 46 (3): 241-80. PMID 6127606. PMC: 281544. 4. Hudault S, Guignot J, Servin AL (July 2001). Gut 49 (1): 47-55. doi:10.1136/gut.49.1.47. PMID 11413110. 5. Reid G, Howard J, Gan BS (September 2001). Trends Microbiol. 9 (9): 424-8. doi:10.1016/S0966-842X(01)02132-1. PMID 11553454. 6. Feng P, Weagant S, Grant, M (2002-09-01). Bacteriological Analytical Manual (8th ed.). FDA/Center for Food Safety & Applied Nutrition. Retrieved on 2007-01-25. 7. Thompson, Andrea (2007-06-04). Taxonomy Browser. NCBI. Retrieved on 2007-11-30. 9. Kubitschek HE (01 January 1990). J. Bacteriol. 172 (1): 94-101. PMID 2403552. PMC: 208405.10. Madigan MT, Martinko JM (2006). Brock Biology of microorganisms (11th ed.). Pearson. ISBN 0-13-196893-9.11. Fotadar U, Zaveloff P, Terracio L (2005). J. Basic Microbiol. 45 (5): 403-4. doi:10.1002/jobm.200410542. PMID 16187264.12. Ingledew WJ, Poole RK (1984). Microbiol. Rev. 48 (3): 222-71. PMID 6387427.13. Darnton NC, etal J Bacteriol. 2007 Mar;18 Br

Additional Info

Recognizes the O antigen of E. coli serotype O157. Non-reactive with E. coli serotypes: O111, O125, O20, O55 & K12.