ESRRG, Control Peptide (Estrogen-related receptor gamma, DKFZp781L1617, ERR3, FLJ16023, KIAA0832, NR3B3)

Internal region, CRMSNKDRHIDS of ESRRG.\n\nControl peptide for E3565-23C.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nTo start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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SPECIFICATIONS

Catalog Number

E3565-23C-P

Size

100ug

Applications

ELISA

Form

Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.

Purity

Highly Purified

References

Genome-wide orchestration of cardiac functions by the orphan nuclear receptors ERRalpha and gamma., Dufour CR, Wilson BJ, Huss JM, Kelly DP, Alaynick WA, Downes M, Evans RM, Blanchette M, Giguere V., Metab. 2007 May;5(5):345-56.

Alternative Names

Estrogen-related receptor gamma; DKFZp781L1617; ERR3; FLJ16023; KIAA0832; NR3B3