b-Estradiol 17-(b-D-glucuronide) (E217G) is a well known cholestatic agent and substrate of multidrug resistance- associated protein 2 (Mrp2), whereas b-estradiol 3-(b-D-glucuronide) (E23G) is a noncholestatic regioisomer of E217G with unknown transport properties.
Ratio:
15-20mol/moles BSA
Storage and stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.