Estrogen Inducible Protein pS2 (Breast Cancer Estrogen Inducible Protein, Breast Cancer Estrogen Inducible Sequence, BCEI, D21S21, Gastrointestinal Trefoil Protein pS2, HP1.A, HPS2, pNR-2, pS2, pS2 Protein, Trefoil Factor 1, TFF1)

pS2 is a cysteine-rich, 6.5kD protein found in both estrogen-dependent (breast tumors) and estrogen-independent tissues (normal stomach mucosa). About 60% of breast carcinomas are positive for pS2. pS2 is primarilyexpressed in estrogen receptor-positive breast tumors and is reportedly useful in identifying a subset of estrogen-dependent breast tumors which may respond to endocrine therapy.\n\nApplications: \nSuitable for use in Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Normal stomach or breast carcinoma tissue sections.\n\nCellular Localization: Cytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E3557-75B

Size

250ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

10mM PBS, pH 7.4, 0.2% BSA

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

Mori K et al. Complete primary structure of the human estrogen-responsive gene (pS2) product. J Biochem (Tokyo) 107:73-6 (1990). <PUBMED:2185238>Rio MC et al. Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa. Science 241:705-8 (1988). <PUBMED:3041593>Jeltsch JM et al. Structure of the human oestrogen-responsive gene pS2. Nucleic Acids Res 15:1401-14 (1987). <PUBMED:3822834>Masiakowski P et al. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res 10:7895-903 (1982). <PUBMED:6897676>

Additional Info

Species Crossreactivity: Crossreacts with Human.