Estrogen Receptor alpha (ER alpha, Estradiol receptor, NR3A1)

Supplier:

United States Biological

Cat no: E3565-10K1

Estrogen Receptor alpha (ER alpha, Estradiol receptor, NR3A1)

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70kD member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators. Human ER alpha is 595aa in length, and contains a DNA binding region (aa185-250), three NLSs (aa256-260; 266-271; 299-303), a steroid-binding site (aa351- 543), a dimerization motif (aa497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3565-10K1

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS) 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human ER alpha at ~66kD.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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