Estrogen Receptor alpha (ER, ER alpha, Era, ESR, ESR, ESR1, ESRA, Estradiol Receptor, Estrogen Receptor, Estrogen Receptor 1)

Supplier:

United States Biological

Cat no: E3565-11Z1

Estrogen Receptor alpha (ER, ER alpha, Era, ESR, ESR, ESR1, ESRA, Estradiol Receptor, Estrogen Receptor, Estrogen Receptor 1)

ERa (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70kD member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ERa is normally quiescent and bound to heat-shock proteins and immunophilins. Following b-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ERb, and binds to DNA with multiple coactivators. Human ERa is 595aa in length. It contains a DNA binding region (aa185-250), three NLSs (aa256-260; 266-271; 299-303), a steroid-binding site (aa351-543), a dimerization motif (aa497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at aa537, aa167 and aa118. Multiple splice forms exist. There is an 80kD isoform that shows a substitution (duplication) of aa412-517 for aa411, a second isoform with a deletion of aa255-366, a third isoform with a deletion of aa152-412, and a fourth isoform that shows a Thr substitution for aa152-595. Human ERa is only 46aa identical to human ERb. Over aa1-116, human ERa shares 85aa identity with mouse ERa.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 8-25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3565-11Z1

Size

100ug

Hosts

Mouse

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2B

Additional Info

Recognizes human ERa. Does not react with recombinant human ERb.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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