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Estrogen Receptor, alpha (ERa)

Estrogen Receptor, alpha (ERa)

Cat no: E3565-09T

Supplier: United States Biological
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Estrogen Receptor alpha is a 65kD protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies. Applications: Suitable for use in Gel Supershift, Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, detects an ~67kD protein representing ER alpha from MCF-7 cell extract. Immunocytochemistry: 10ug/ml Immunohistochemistry (Paraffin-embedded sections): 5ug/ml, staining of ER alpha in mouse Leydig cells results in both nuclear and cytoplasmic staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E3565-09T
Reactivities: Human, Mouse, Rat, Bovine
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purifed by immunoaffinity chromatography.
References: 1. J. Bone Mineral Res. 8: 1103-1109 (1993). 2. Biochem. Biophys. Res. Comm. 195: 730-736 (1993). 3. Steroids 61: 549-556 (1996). 4. Steroids 55: 196-208 (1990). 5. Science 273: 1222-1225 (1996). 6. Blood 87: 2683-2692 (1996). 7. J. Bone Mineral Res. 7: S269 (1992). 8. Biol. Reprod. 68: 1959-1967 (2003). 9. Toxicological Sciences 68: 389-402 (2002). 10. Tox. Sci. 68: 389-402 (2002). 11. Invest. Ophthalmol. Vis. Sci. 39: 2105 (1998). 12. J. Biol. Chem. 282: 10,146-10,152 (2007).
Additional info: Recognizes both the steroid occupied and unoccupied forms of human estrogen receptor (ER) alpha at ~65kD. Does not crossreact with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor. Species Crossreactivity: rat, mouse, bovine.

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