Estrogen Receptor, alpha (ERa)

Estrogen Receptor alpha is a 65kD protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription.\nLike other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.\n\nApplications: \nSuitable for use in Gel Supershift, Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml, detects an ~67kD protein representing ER alpha from MCF-7 cell extract.\nImmunocytochemistry: 10ug/ml\nImmunohistochemistry (Paraffin-embedded sections): 5ug/ml, staining of ER alpha in mouse Leydig cells results in both nuclear and cytoplasmic staining.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-09T

Size

50ug

Applications

FC, ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purifed by immunoaffinity chromatography.

Isotype

IgG1

References

1. J. Bone Mineral Res. 8: 1103-1109 (1993). 2. Biochem. Biophys. Res. Comm. 195: 730-736 (1993). 3. Steroids 61: 549-556 (1996). 4. Steroids 55: 196-208 (1990). 5. Science 273: 1222-1225 (1996). 6. Blood 87: 2683-2692 (1996). 7. J. Bone Mineral Res. 7: S269 (1992). 8. Biol. Reprod. 68: 1959-1967 (2003). 9. Toxicological Sciences 68: 389-402 (2002). 10. Tox. Sci. 68: 389-402 (2002). 11. Invest. Ophthalmol. Vis. Sci. 39: 2105 (1998). 12. J. Biol. Chem. 282: 10,146-10,152 (2007).

Additional Info

Recognizes both the steroid occupied and unoccupied forms of human estrogen receptor (ER) alpha at ~65kD. Does not crossreact with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor. Species Crossreactivity: rat, mouse, bovine.