Estrogen Receptor, alpha, phosphorylated (Ser167) (ER-alpha, ERa, ER, ESR1, ESR, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)

Estrogen receptor a (ERa), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, (espectively), ERa regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ERa activity (3,4). ERa is phosphorylated on multiple sites (5). Ser104, 106, 118 and 167 are located in the aminoterminal transcription activation function domain AF-1, and\nphosphorylation of these serines plays an important role in regulating ERa activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-10A1

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Mangelsdorf, D.J. et al. (1995) Cell 83, 835-839. (2) Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev. 14, 121-141. (3) Chen, D. et al. (1999) Mol. Cell. Biol. 19, 1002-1015. (4) Campbell, R.A. et al. (2001) J. Biol. Chem. 276, 9817-9824. (5) Chen, D. et al. (2000) Mol. Cell 6, 127-137. (6) Joel, P.B. et al. (1998) Mol. Cell. Biol. 18, 1978-1984.

Additional Info

Recognizes endogeneous levels of ERa, phosphorylated at (Ser167). Species Crossreactivity: mouse.