Home  >  Products  >  Estrogen Receptor, alpha, phosphorylated (Ser167) (ER-alpha, ERa, ER, ESR1, ESR, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)
Estrogen Receptor, alpha, phosphorylated (Ser167) (ER-alpha, ERa, ER, ESR1, ESR, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)

Estrogen Receptor, alpha, phosphorylated (Ser167) (ER-alpha, ERa, ER, ESR1, ESR, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)

Cat no: E3565-10A1

Supplier: United States Biological
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Estrogen receptor a (ERa), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, (espectively), ERa regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ERa activity (3,4). ERa is phosphorylated on multiple sites (5). Ser104, 106, 118 and 167 are located in the aminoterminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ERa activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E3565-10A1
Reactivities: Human, Non-Human Primate
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
References: (1) Mangelsdorf, D.J. et al. (1995) Cell 83, 835-839. (2) Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev. 14, 121-141. (3) Chen, D. et al. (1999) Mol. Cell. Biol. 19, 1002-1015. (4) Campbell, R.A. et al. (2001) J. Biol. Chem. 276, 9817-9824. (5) Chen, D. et al. (2000) Mol. Cell 6, 127-137. (6) Joel, P.B. et al. (1998) Mol. Cell. Biol. 18, 1978-1984.
Additional info: Recognizes endogeneous levels of ERa, phosphorylated at (Ser167). Species Crossreactivity: mouse.

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