Estrogen Receptor alpha, Recombinant, Human (ERa)

The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Recombinant human ER alpha protein , aa1-300.\n\nApplications:\nSuitable for use in ELISA, Inhibition Assays and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researacher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

E3565-10R

Size

2ug

Applications

ELISA, WB

Reactivities

Hum

Form

Supplied as a sterile-filtered liquid in 50mM Tris-HCl, pH 7.5, and 10mM reduced glutathione.

Purity

Purified

References

1. Estrogen Receptor-{alpha} Methylation Predicts Melanoma Progression. Cancer Res 2006 Jul 1;66(13):6692-8. 2. Vitamin D and estrogen receptor-alpha genotype and indices of bone mass and bone turnover in Danish girls. J Bone Miner Metab 2006;24(4):329-36. 3. Impact of Estrogen Receptor Beta on Gene Networks Regulated by Estrogen Receptor Alpha in Breast Cancer Cells. Endocrinology 2006 Jun 29. 4. A mixture model based discriminate analysis for identifying ordered transcription factor binding site pairs in gene promoters directly regulated by estrogen receptor-{alpha} Bioinformatics 2006 Jun 29. 5. The Forkhead box M1 protein regulates the transcription of the estrogen receptor alpha in breast cancer cells. J Biol Chem 2006 Jun 28. 6. Down-regulation of estrogen receptor-alpha in MCF-7 human breast cancer cells after proteasome inhibition. Biochem Pharmacol 2006 Jun 26.