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Estrogen Receptor, beta (ERb)

Estrogen Receptor, beta (ERb)

Cat no: E3565-16T

Supplier: United States Biological
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The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domains have diverged significantly. Similar in function to ER-alpha, ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's). Positive Control: Recombinant fusion protein. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3565-16T
Reactivities: Human
Hosts: Mouse
Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: As reported
P type: Mab
Isotype: IgG2b,k
Purity: Purified by Protein G affinity chromatography.
References: 1. Mosselman, S., (1996) FEBS Letter. 392:49-53. 2. Dotlaw, H., et al., (1996) J. Clin. Endo. Metab. 82:2371-2374. 3. Kuiper, G.G.J.M., et al., (1997) Endocrinology 138:863-870.
Additional info: Specific for human Estrogen receptor-Beta.

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