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Estrogen Receptor, beta (ERb)

Estrogen Receptor, beta (ERb)

Cat no: E3565-16W

Supplier: United States Biological
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Applications: Suitable for use in Western Blot, Immunohistochemistry (paraffin), and Gel shift assay. Other applications not tested. Recommended Dilution: Gel Shift: 1-5ug/ml Immunohistochemistry: 1-2ug/ml.. IH staining of ER beta in rat cerebellum results in cytoplasmic staining in Purkinje neurons. Western Blot: 1-2ug/ml. In rat brain homogenate, detects an ~55kD protein representing ER beta Optimal dilutions to be determined by the researcher. Cellular Localization: Nuclear Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3565-16W
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, containing 1mg/ml BSA and 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Rosenfeld CS et al. (1999) Biol Reprod 60:691-7. 2. Rosenfeld CS et al. (1998) Endocrinology 139:2982-7. 3. Makela S et al. (1999) Proc Natl Acad Sci USA 96:7077-82. 4. Alves SE et al. (1998) Proc Natl Acad Sci USA 95:3281-6. 5. Krege JH et al. (1998) Proc Natl Acad Sci USA 95:15677-82. 6. Petersen DN et al. (1998) Endocrinology 139:1082-92. 7. Li X et al. (1997) Neuroendocrinology 66:63-7.
Additional info: Recognizes human estrogen receptor beta. Species Crossreactivity: rat, mouse and primate.

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