Estrogen Receptor, beta (ERb)

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Estrogen receptor (ER) was first cloned in 1987 and has been shown to be expressed and transcriptionally active in a number of tissues including ovary, testis, prostate and brain. Until recently, only the "classical" ER (ER alpha) has been known. However, a new estrogen binding receptor, referred to as ER beta, has recently been cloned in rat and humans.\nER beta has been shown to be a high affinity estrogen binding protein capable of initiating transcription of genes under the control of estrogen response elements (ERE)\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Paraffin): 2-5ug/ml. Perform heat mediated antigen retrieval using citrate buffer.\nImmunohistochemistry (Frozen): 2-5ug/ml\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-16Z

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

References

1. Alves SE et al. (1998) Proc Natl Acad Sci USA 95:3281- 2. Duong, B. N., et al. (2006) AKT Regulation of Estrogen Receptor {beta} Transcriptional Activity in Breast Cancer, 66, 8373-8381.

Additional Info

Recognizes human estrogen receptor (ER) beta at ~55kD. Species Crossreactivity: rat