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Estrogen Receptor, beta (ERb, ER-beta, Nuclear Receptor Subfamily 3 Group A Member 2, ESR2, ESTRB, NR3A2)

Cat no: E3558-40C

Estrogen Receptor, beta (ERb, ER-beta, Nuclear Receptor Subfamily 3 Group A Member 2, ESR2, ESTRB, NR3A2)

Estrogen Receptor (ER) exists in two types: alpha and beta. They are similar in structure. The ligand binding domain and the DNA binding domain are highly conserved in these proteins while the N-terminal transactivating domain is considerably diverged. Five isoforms of the hER beta gene, designated hER b 1-5 have been identified. ER-b binds to estrogen and activates the gene through direct interaction with estrogen specific gene elements (ERE's). ER-b RNA has been detected in human thymus, spleen, ovary and testis and in rat ovary and prostrate. The tissue distribution overlaps but is not identical to that of ER-a RNA. ER-b knock out mice show severe infertility.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF-7 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3558-40C

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human Estrogen Receptor beta. Species Crossreactivity: rat.

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