Estrogen Receptor beta5 (ERb5)

Eestrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription.\nImmunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied (1). Studies also revealed the nuclear expression of ERB5 in the majority of breast carcinomas tested, with a possible association with improved patient survival (2).\n\nApplications: \nSuitable for use in Western Blot. and Immunohistochemistry Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Paraffin): Requires antigen retrieval using sodium citrate buffer pH 6.0 heat treatment\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nNormal colon or colon carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E3565-17Z

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G Chromatography.

Isotype

IgG1

References

1. Wong, N. et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates. J. Pathol. 207: 53-60. 2. Shaaban, A.M. et al. (2008) Nuclear and cytoplasmic expression of ERbeta1, ERbeta2, and ERbeta5 identifies distinct prognostic outcome for breast cancer patients. Clin. Cancer Res. 14: 5228-5235.

Additional Info

Recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5).