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Estrogen Receptor (BSA & Azide Free) (ER)

Estrogen Receptor (BSA & Azide Free) (ER)

Cat no: E3564-89X

Supplier: United States Biological
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ER protein exhibits six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. It is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors may improve response to anti-estrogen therapy. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2 hours at RT ELISA: Use as a reporter antibody Optimal dilutions to be determined by the researcher Positive Control: MCF-7 cells, Breast Carcinoma Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E3564-89X
Reactivities: Human, Rat
Hosts: Rat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See E3564-89.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein G affinity chromatography.
References: 1. Green G, et al. (1984) J. Steroid Biochem. 20:51-56. 2. Katzenellenbogen B, et al. (1987) Biochemistry 26:2364-2373. 3. Miller R T, Hapke M R, Greene G L. (1993) Cancer 71:3541-3546.
Additional info: Recognizes human Estrogen Receptor alpha at ~67kD. Species Crossreactivity: rat

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