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Estrogen Receptor (ER) (BSA & Azide Free)

Cat no: E3564-80X

Estrogen Receptor (ER) (BSA & Azide Free)

Applications: Gel Supershift (1mg/ml), Immunoprecipitation. Not recommended for Immunohistochemistry, Western Blotting.\n\nRecommended Dilution:\nImmunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate\nOptimal dilutions to be determined by the researcher.

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SPECIFICATIONS

Catalog Number

E3564-80X

Size

100ug

Applications

IP

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Prc

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.\nAlso available with BSA and azide, cat# E3564-80.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Green S; Chambon P. (1986) Nature 324(6098):615-7. 2. Green S; Gronemeyer H; et al. (1987) Chichester, England, Ellis Horwood Ltd. 7-28. 3. Evans RM, (1988) Science 240:889-95. 4. Danielson M; Northrop JP; et al. (1986) EMBO Journal 5:2513-22. 5. Kumar V, et al., (1987) Cell 51(6):941-51.\n6. Greene GL; Sobel BN; et al., (1988) Molecular Endocrinology 2:714-26. 7. Jensen EV; Jacobson HI. (1962) Recent Progress in Hormone Research 18:387-414. 8. Walter P, et al., (1985) Proc Nat Academy of Sciences of the United States of America 82(23):7889-93. 9. Green S, et al., (1986) Journal of Steroid Biochemistry 24(1):77-83. 10. Greene GL, et al., (1986) Science 231(4742):1150-4.

Additional Info

Recognizes Estrogen Receptor at 67kDa. The epitope maps between residues 302-553 of the E domain of human ER. This antibody blocks binding of estrogen to ER. Species Reactivity: Human, bovine, porcine, canine, Mouse, Rat, Hamster and Chicken.

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