Estrogen Receptor (ER)

Applications: \nSuitable for use in Immunoprecipitation, Immunohistochemistry (paraffin sections), and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin/paraffin): 1:40-1:80 for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.\nImmunoprecipitation (denatured verified): 10ul/mg protein lysate. Use Protein G.\nWestern Blot: 1:50-1:100 for 2 hrs. at RT\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3564-82

Size

500ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as concentrated tissue culture supernatant in 15mM sodium azide.

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG1

References

1. Green S; Chambon P. (1986) Nature 324(6098):615-7. 2. Green S; Gronemeyer H; et al. (1987) Chichester, England, Ellis Horwood Ltd. 7-28. 3. Evans RM, (1988) Science 240:889-95. 4. Danielson M; Northrop JP; et al. (1986) EMBO Journal 5:2513-22. 5. Kumar V, et al., (1987) Cell 51(6):941-51.\n6. Greene GL; Sobel BN; et al., (1988) Molecular Endocrinology 2:714-26. 7. Jensen EV; Jacobson HI. (1962) Recent Progress in Hormone Research 18:387-414. 8. Walter P, et al., (1985) Proc Nat Academy of Sciences of the United States of America 82(23):7889-93. 9. Green S, et al., (1986) Journal of Steroid Biochemistry 24(1):77-83. 10. Greene GL, et al., (1986) Science 231(4742):1150-4.

Additional Info

Recognizes human Estrogen Receptor at 67kDa. Species Reactivity: Rat.