Estrogen Receptor (ER)

Estrogen Receptor alpha is a 65kD protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:250\nImmunoprecipitation: 15ug/ml only Immunoprecipates the active receptor.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-06L

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Mueller SO & Korach KS (2001) Curr Opin Pharmacol 1:613-9. 2. Olefsky JM (2001) J Biol Chem 276:36863-4. 3. Moggs JG & Orphanides G (2001) EMBO Rep 2:775-81. 4. Wyckoff MH et al. (2001) J Biol Chem 276:27071-6. 5. Couse JF et al. (1997) Endocrinology 138:4613-21.

Additional Info

Recognizes human estrogen receptor. Does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor.