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Estrogen Receptor (ER, ER-alpha, Era, ESRA, Estradiol Receptor, ESR, ESR1, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)

Cat no: E3565-06M

Estrogen Receptor (ER, ER-alpha, Era, ESRA, Estradiol Receptor, ESR, ESR1, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)

Estrogen receptor (ER) is a ligand activated, transactivating protein. The wild type ER is a 66kD protein coded by a 6.6kb mRNA, comprised of five functional domains derived from eight exons. The three domains of the ER which show the greatest degree of sequence homology are the A domain which, in concert with the B domain, contains the transactivation function (AF-1), the C domain which contains two zinc-fingers and is responsible for DNA binding and the E domain which is responsible for ligand binding as well as the ligand inducible transactivation function (AF-2). It maps to 6q25.1 region of Human chromosome.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBreast carcinoma\n\nCellular Localization: \nNuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-06M

Size

200ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Pink et al. Nucleic Acids Res 24: 962, 1996.

Additional Info

Recognizes human Estrogen Receptor. Species Crossreactivity: hamster, mouse and rat.

Alternative Names

Estrogen Receptor 1, DKFZp686N23123

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