Estrogen-Related Receptor, alpha (ERRa, NR3B1, NR3 Steroid Receptor)

Supplier:

United States Biological

Cat no: E3565-13G

Estrogen-Related Receptor, alpha (ERRa, NR3B1, NR3 Steroid Receptor)

ERR alpha is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has also been shown to interact with estrogen and the transcription factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD),and thyroid hormone receptor genes. ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in brain, spinal cord, pituitary gland and heart. More recently, ERR alpha has been observed in various human breast cancer cell lines, breast adipose tissue and breast tumors. Studies demonstrating the use of ERR alpha antagonists in treating breast cancer have excited great interest in this receptor in the search for curing the disease (Chisamore, 2009).\n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nImmunocytochemistry: 1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3565-13G

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG

Additional Info

Recognizes human ERR-alpha P3. Species sequence homology: mouse, rat.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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