The B-Bridge Estrone-3-Glucuronide (E1G) Immunoassay kit uses a specifically generated antibody to measure E1G and its metabolites in urine and fecal samples, or in extracted serum and plasma. The kit will quantitatively measure E1G present in diluted buffer samples and tissue culture media samples. An E1G standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1G-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1G to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1G-peroxidase conjugate. After a short incubation, the reaction is read at 450nm wavelength.
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