Eukaryotic transcriptome sequencing can be divided into reference-based and non-reference-based scenarios. Among these, non-reference-based sequencing refers to analyzing the eukaryotic transcriptome without a reference genome. For Eukaryotic Non-ginseng Transcriptome Sequencing, the raw data must first undergo quality control and be assembled into unigenes. These unigenes are then used as reference sequences for subsequent analyses, including functional annotation, SNP, and SSR marker development.
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