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Eukaryotic Translation Initiation Factor 2 alpha Kinase 4, phosphorylated (Thr899) (eIF2AK4, GCN2, General Control Nonderepressible 2)

Eukaryotic Translation Initiation Factor 2 alpha Kinase 4, phosphorylated (Thr899) (eIF2AK4, GCN2, General Control Nonderepressible 2)

Cat no: E8949-18B

Supplier: United States Biological
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In eukaryotic cells, protein synthesis is regulated in response to various environmental stresses by phosphorylating the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha). Four different eIF2alpha kinases have been identified in mammalian cells, the heme-regulated inhibitor (HRI), the interferon-inducible RNA-dependent kinase (PKR) and the endoplasmic reticulum-resident kinase (PERK) and GCN2, which was previously characterized from Saccharomyces cerevisiae, Drosophila melanogaster and Neurospora crassa. GCN2 is the unique eIF2alpha kinase present in all eukaryotes from yeast to mammals. GCN2, a sensor of amino acid deficiency, plays a key role in yeast and mammals in modulating amino acid metabolism as part of adaptation to nutrient deprivation. Results demonstrate that PERK and GCN2 function to cooperatively regulate eIF2alpha phosphorylation and cyclin D1 translation after unfolded protein response pathway activation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: E8949-18B
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Berlanga JJ, et al. Eur J Biochem 265(2):754-62 2. Guo F, et al. Cell Metab 5(2):103-14, 2007 3. Hamanaka RB, et al. Mol Bio Cell 16(12):5493-501, 2005
Additional info: Recognizes human Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 phosphorylyated at threonine 899.

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