Eukaryotic Translation Initiation Factor 2B, epsilon (eIF2B-e)

Supplier:

United States Biological

Cat no: E8949-20

Eukaryotic Translation Initiation Factor 2B, epsilon (eIF2B-e)

Phosphorylation of the a subunit of eukaryotic initiation factor 2 is a well documented mechanism of down regulating protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B (1,2). Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the a subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex, inhibiting the turnover of eIF2B. Induction of PKR by IFN-g and TNF-a, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2a at Ser51 (5,6). eIF2B, a guanine nucleotide exchange factor, is composed of 5 subunits, the largest of which is eIF2B-e (7). Multiple in vivo phosphorylation sites have been identified on eIF2B-e (8). Casein Kinase II can phosphorylate eIF2B-e at Ser717/718 to allow for association with its substrate eIF2. Phosphorylation at Ser544 allows GSK-3 to phosphorylate the key regulatory site Ser540. A fifth eIF2B-e phosphorylation site, Ser466, can be phosphorylated by casein kinase I.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E8949-20

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1.Kimball, S.R., (1999) Int. J. Biochem. Cell Biol. 31:25

Additional Info

Recognizes human endogenous levels of total eIF2B-e protein. Species Crossreactivity: Mouse, rat and monkey.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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