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Eukaryotic Translation Initiation Factor 3, Subunit 12 (eIF3S12, ARG134, eIF3k, HSPC029, MSTP001, M9, PLAC-24, PRO1474, PTD001)

Eukaryotic Translation Initiation Factor 3, Subunit 12 (eIF3S12, ARG134, eIF3k, HSPC029, MSTP001, M9, PLAC-24, PRO1474, PTD001)

Cat no: E8949-37P

Supplier: United States Biological
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Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004 [PubMed 14688252]; Fraser et al., 2007 [PubMed 17588516]).[supplied by OMIM] Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2000-1:10000 Immunoprecipitation: 2-5ug/mg lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E8949-37P
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: eIF-3 p25, eIF-3 p28, Muscle-specific Gene M9 Protein
Additional info: Recognizes human EIF3S1.

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