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Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1, 4E-T, Clast4, eIF4E Transporter, FLJ21601)

Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1, 4E-T, Clast4, eIF4E Transporter, FLJ21601)

Cat no: E8949-45L

Supplier: United States Biological
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eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. . Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000. Immunoprecipitation: 2-5ug/mg lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E8949-45L
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Alternative names: EIF4ENIF1, 4E-T, Clast4, eIF4E Transporter, FLJ21601
Additional info: Species Crossreactivity: Human and mouse. Other species have not been tested.

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