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Exendin 4 (Biotin)

Cat no: E9050-03C1

Exendin 4 (Biotin)

Exendin-4 is a 39aa peptide found in venom from the Gila monster Heloderma suspectum (1). It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion (2).\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E9050-03C1

Size

100ul

Applications

ELISA

Hosts

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in 0.01M phosphate buffer, pH 7.4, 0.14M NaCl and 15mM sodium azide. Labeled with Biotin.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

References

1. Eng J, Kleinman WA, Singh L, Singh G, Raufman JP (1992) Isolation and characterization of exendin-4, an exendin-3 analogue, from Heloderma suspectum venom. Further evidence for an exendin receptor on dispersed acini from guinea pig pancreas. J Biol Chem 267:7402-7405. 2. Goke R, Fehmann HC, Linn T, Schmidt H, Krause M, Eng J, Goke B (1993) Exendin-4 is a high potency agonist and truncated exendin-(9-39)-amide an antagonist at the glucagon-like peptide 1-(7-36)-amide receptor of insulin-secreting beta-cells. J Biol Chem 268:19650-19655.

Additional Info

Recognizes exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.

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