Extractable Nuclear Antigen, Human, IgG, IgA, IgM BioAssay(TM) ELISA Kit (ENA)

The United States Biological Extractable Nuclear Antigen, Human, IgG, IgA, IgM BioAssay(TM) ELISA Kit is intended for the detection of antibodies to Sm, RNP, Ro, La, Scl-70, and Jo-1 in human sera. The assay is to be used to detect antibodies in a single serum specimen. The results of the assay are to be used as an aid to the research of systemic lupus erythem atosis, mixed connective tissue disease, Sjogren syndrome, scleroderma, polymyositis, and dermatomyositis. This assay does not differerentiate these conditions.\n\nSystematic autoimmune disease is characterized by the presence of circulating autoantibodies directed to a wide variety of cellular antigens. Systemic lupus erythematosis (SLE), commonly referred to as Lupus, is the best known of these diseases. Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, and polymyositis/dermatomyositis. \n\nThe Sm (Smith) antigen is composed of nuclear RNA and several polypeptides. Antibodies to Sm are present in approximately 30% of patients with SLE. Sm is a very specific marker for SLE. Sm antibodies are very rare in other autoimmune diseases and normals.\n\nRNP antigen is composed of RNA and several protein components. The antigen is closely associated with Sm antigen. Approximately 45% of patients with SLE have antibodies to RNP. RNP antibodies are also found in patients with discoid lupus, Sjogren syndrome, scleroderma, rheumatoid arthritis and MCTD. Very high titers to RNP is a characteristic of MCTD.\n\nRo (SS-A) antigen consists of protein complexed with RNA. Approximately 60% of patients with Sjogren syndrome and 35% of SLE patients have antibodies to Ro. SLE patients with Ro antibodies alone (negative La/SSB) have a greater chance of developing nephritis.\n\nLa (SS-B) antigen consists of a protein complexed to RNAs. Approximately 60% of patients with Sjogren syndrome and 15% of SLE patients have antibody to La. Ro (SSA) antibody occurs in virtually all sera with La antibody. However, some SLE patients have Ro only antibody profiles.\n\nScl-70 antigen is a nuclear enzyme topoisomerase 1. Approximately 20-30% of patients with scleroderma have antibodies to Scl-70. The antibody is rarely seen in other conditions. Scl-70 antibodies are usually present in the diffuse subtype of scleroderma while antibodies to centromere proteins are dominant in the limited subtype of scleroderma or CREST syndrome.\n\nJo-1 antigen is the cellular enzyme histidyl-tRNA synthetase. Approximately 30% of patients with polymyositis (PM) and 10% of patients with dermatomyositis (DM) have antibodies to Jo-1. Antibodies to Jo-1 are seen rarely in normals and other diseases except for those which overlap with PM-DM.\n\nClassically, antibodies to autoimmune antigens are detected by double immunodiffusion. However, the test is lengthy and suffers weak sensitivity. ELISAs combine greater sensitivity with ease of use. Many ELISAs have been developed and validated for detecting autoantibodies to various antigens.\n\nKit Components:\n1. Sm, RNP, Ro, La, Scl-70, Jo-1 antigen coated microassay plate 1x96 wells\n2. Serum Diluent, 1x30ml. Supplied as a liquid in buffer, BSA, Tween-20, 0.1% proclin. \n3. Positive Control, High, 1x400ul. Supplied as a liquid in < 0.1%, sodium azide, 0.01% pen/strep.\n4. Calibrator, 1x400ul. Supplied as a liquid in in < 0.1%, sodium azide, 0.01% pen/strep. \n5. Negative Control, 1x400ul. Supplied as a liquid in < 0.1%, sodium azide, 0.01% pen/strep.\n6. Positive Control, Low, 1x400ul. Supplied as a liquid in < 0.1%, sodium azide, 0.01% pen/strep.\n7. IgG, IgA, IgM (HRP) Goat x human, 1x15ml. Supplied as a liquid in 0.1% proclin, gentamicin.\n8. Wash Buffer, 20X, 1x50ml. Dilute 1 part concentrate + 19 parts ddH2O. Supplied as a liquid in TBS, Tween-80, 0.1% proclin.\n9. TMB (Tetramethylbenzidine), 1x15ml. The reagent should remain closed when not in use. If allowed to evaporate, a precipitate may form in the reagent wells. \n10. Stop Solution, 1x15ml. Supplied as a liquid 1N H2SO4 solution. \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E9012

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

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