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EZH2 (Enhancer of Zeste Homolog 2, Histone-lysine N-methyltransferase EZH2, ENX1, ENX-1, KMT6, KMT6A, Lysine N-methyltransferase 6, MGC9169)

EZH2 (Enhancer of Zeste Homolog 2, Histone-lysine N-methyltransferase EZH2, ENX1, ENX-1, KMT6, KMT6A, Lysine N-methyltransferase 6, MGC9169)

Cat no: E9085-02D

Supplier: United States Biological
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The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-richaa stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Chromatin IP. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:200 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50 Immunoprecipitation: 1:200 Chromatin IP: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E9085-02D
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
Alternative names: EC=2.1.1.43
References: (1) Seller, W.B. and Loda, M. (2002) Cancer Cell 2, 349-350. (2) Visser, H.P. et al. (2001) Br. J. aematol. 112, 950-958. (3) Chen, H. et al. (1996) Genomics 38, 30-37. (4) Tonini, T. et al. (2004) Oncogene 23, 4930-4937. (5) Muller, J. et al. (2002) Cell 111, 197-208. (6) Kleer, C.G. et al. (2003) Proc Natl. Acad. Sci. USA 100.
Additional info: Recognizes endogenous levels of total Ezh2. Species Crossreactivity: mouse, rat, monkey.

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