EZH2 (Enhancer of Zeste Homolog 2, Histone-lysine N-methyltransferase EZH2, ENX1, ENX-1, KMT6, KMT6A, Lysine N-methyltransferase 6, MGC9169)

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large\nprotein family, contains four conserved regions including domain I, domain II, and a cysteine-richaa stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). \n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Chromatin IP. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (IF-IC): 1:200\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,\n0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunohistochemistry (Paraffin): 1:50\nImmunoprecipitation: 1:200\nChromatin IP: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E9085-02D

Size

100ul

Applications

IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Seller, W.B. and Loda, M. (2002) Cancer Cell 2, 349-350. (2) Visser, H.P. et al. (2001) Br. J. aematol. 112, 950-958. (3) Chen, H. et al. (1996) Genomics 38, 30-37. (4) Tonini, T. et al. (2004) Oncogene 23, 4930-4937. (5) Muller, J. et al. (2002) Cell 111, 197-208. (6) Kleer, C.G. et al. (2003) Proc Natl. Acad. Sci. USA 100.

Additional Info

Recognizes endogenous levels of total Ezh2. Species Crossreactivity: mouse, rat, monkey.

Alternative Names

EC=2.1.1.43