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Ezrin (Cytovillin, Villin-2, p81, EZR, VIL2)

Cat no: E9100-25H

Ezrin (Cytovillin, Villin-2, p81, EZR, VIL2)

Ezrin is a member of the ERM family (ezrin, radixin, moesin) of proteins that link the cell membrane to the actin cytoskeleton and that are involved in cytoskeletal organization, cell motility and morphogenesis. The protein (586aa) consists of three domains: a membrane-binding aminoterminal domain, an extended alpha-helical domain, and a carboxyterminal domain that has an actin-binding site. Aberrantly high ezrin protein expression has been detected in the stromal cells of hemangioblastoma. Moreover, a high ezrin expression level is associated with immortalization and the increased proliferation rate of cells in vitro. It appears to play a role in the progression of tumors, such as the metastasis of osteosarcoma. Human Ezrin gene is localized to the end of the long arm of chromosome 6 (6q22-q27).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nA431 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E9100-25H

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Markku, K. et al. Hum. Genet. 103:662-665 (1998). 2. Turunen, OT. et al. J. Cell. Biol. 126:1445-1445 (1994).

Additional Info

Recognizes human Ezrin. Species Crossreactivity: mouse and rat.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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