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Ezrin, phosphorylated (Tyr353) (Cytovillin, Villin-2, p81, EZR, VIL2)

Ezrin, phosphorylated (Tyr353) (Cytovillin, Villin-2, p81, EZR, VIL2)

Cat no: E9100-25J

Supplier: United States Biological
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The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts their amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E9100-25J
Reactivities: Human, Non-Human Primate
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1.Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510. 2.Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192. 3.Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657. 4.Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203. 5.Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576. 6.Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.
Additional info: Recognizes endogenous levels of human Ezrin, phosphorylated at Tyr353. Species Crossreactivity: Monkey.

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