Ezrin (Thr567), Radixin (Thr564), Moesin (Thr558), phosphorylated, Blocking Peptide

The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts their amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).\n\nDescription:\nThis peptide is used to block Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Rabbit mAb reactivity.\n\nQuality Control:\nThe quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Rabbit mAb by immunohistochemistry.\n\nApplications:\nUse as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. Other applications not tested. \n\nDirections for Use:\nFor immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E9100A

Size

100ug

Applications

IHC

References

1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274:34507-34510. 2. Mangeat, P. et al. (1999) Trends Cell Biol. 9:187-192. 3. Matsui, T. et al. (1998) J. Cell Biol. 140:647-657. 4. Gautreau, A. et al. (2000) J. Cell Biol. 150:193-203. 5. Tran Quang, C. et al. (2000) EMBO J. 19:4565-4576. 6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:7300-7305.